To provide a procedure for operating the UV – VIS Spectrophotometer.
This covers the procedure for operating UV–VIS Spectrophotometer.
MODEL : UV-1700 with PC software
MAKE : Shimadzu.
6.1. For PHOTOMETRIC
6.1.1. Switch on the instrument and computer
6.1.2. Check the connection cable to the PC is properly connected or not.
6.1.3. Select the icon of UV probe and click double
6.1.4. Press PC control (F4) in the instrument.
6.1.5. Select the icon Photometry.
6.1.6. Click the CONNECT icon.
6.1.7. Place the blank solution in sample compartment
6.1.8. Click the method icon(M).The page will be opened enter the wavelength & add the parameter, select calibration and enter raw data and close the window and method should be saved in method folder( which is suitable for name of the sample).
6.1.9. Keep blank and perform AUTOZERO
6.1.10. To read the samples keep the test solution and enter the sample ID in sample table and click the Read unknown icon.(If the method is already exists open the method file place the blank and make AUTOZERO).
6.1.11. To take print out click on REPORT icon and go to file open , select “ B & B PHOTOMETRIC DISSOLUTION” .Change the necessary information and save the report in data folder as XYZ. Where X is name of the sample or code, Y is batch No & Z is date, month & data.
6.1.12. Then preview the report and give the print out command.
6.2. FOR SPECTRUM
6.2.1. Switch on the instrument and computer.
6.2.2. Check the connection cable to the PC is properly connected or not.
6.2.3. Select the icon of UV probe and click double.
6.2.4. Press PC control (F4) in the instrument.
6.2.5. Select the icon Spectrum and click.
6.2.6. Place the blank solution in sample compartment
6.2.7. Click the method icon(M).The page will be opened, enter the wavelength range & scan at medium speed, and enter the necessary information which are relevant then click and the method should be saved as name of the sample and do the base line correction. (If the method is already exists open the method file , place the blank and do the base line correction).
6.2.8. To read the sample spectrum place the sample solution in sample compartment and click the START icon.
6.2.9. After spectrum scanning completion new data set will appear on screen automatically. Enter the file Name :dzu\uvprobe\data\xyz where X is sample name or code, Y is B.No , Z is date and Month and data, data storage to be same enter the analyst name and enter the analyst name and comment if required, click ok and should be saved
6.2.10. After completion of scanning go to operation click on peak pick, pick table will appear on monitor.
6.2.11. To take print out click on REPORT icon and go to file open select “ B& B SPECTRUM PEAK PICK”
6.2.12. Change the necessary information allotted space and save the report
6.2.13. Then preview the report and then give print out command.